郑州大学第一附院AJCR图片被质疑，作者已回复

标题：MiR-210-3p inhibits the tumor growth and metastasis of bladder cancer via targeting fibroblast growth factor receptor-like 1

期刊：American Journal of Cancer Research

单位：郑州大学第一附属医院

发表时间：2017年8月1日

Pubmed: 28861329

研究摘要：

Current evidence indicates that microRNAs are widely down-regulated in various tumors including colorectal carcinoma, liver cancer and lung cancer, and function as tumor suppressors through inhibiting cancer cell growth, invasion and migration. Here, we demonstrated that miR-210-3p level was significantly reduced in the bladder cancer compared to paratumor tissues, and attempt to reveal the regulatory role of miR-210-3p in bladder cancer progression. Exogenous overexpression of miR-210-3p inhibited the proliferation, migration and invasion of bladder cancer cells in vitro. In addition, the nude mouse xenograft model showed that miR-210-3p over-expressing inhibited bladder cancer growth and liver metastasis whereas silencing miR-210-3p caused an opposite outcome, which is mainly regulated by targeting fibroblast growth factor receptor-like 1 (FGFRL1). We also demonstrated that the expression of FGFRL1 in bladder cancer specimens were negatively correlated with miR-210-3p level, and FGFRL1 overexpression rescued the cell proliferation and invasion inhibited by ectopic expression of miR-210-3p. Moreover, knockdown of FGFRL1 was able to mimic the cell growth and metastasis effects induced by miR-210-3p over-expressing in bladder cancer cells. Together, these results indicate that miR-210-3p plays an important role in the regulation of bladder cancer growth and metastasis in vitro and in vivo through targeting FGFRL1.

当前证据表明，microRNAs 在包括结直肠癌、肝癌和肺癌在内的多种肿瘤中广泛下调，通过抑制癌细胞生长、侵袭和迁移发挥肿瘤抑制因子作用。本研究中，我们证明了与肿瘤旁组织相比，膀胱癌中 miR-210-3p 水平显著降低，并试图揭示 miR-210-3p 在膀胱癌进展中的调控作用。体外实验中，外源性过表达 miR-210-3p 抑制了膀胱癌细胞的增殖、迁移和侵袭。此外，裸鼠异种移植模型显示，过表达 miR-210-3p 抑制了膀胱癌的生长和肝转移，而沉默 miR-210-3p 则产生相反的结果，这主要是由靶向成纤维细胞生长因子受体样 1（FGFRL1）调节的。我们还证明了膀胱癌标本中 FGFRL1 的表达与 miR-210-3p 水平呈负相关，FGFRL1 过表达可挽救由 miR-210-3p 异位表达抑制的细胞增殖和侵袭。 此外，敲低 FGFRL1 能够模拟 miR-210-3p 在膀胱癌细胞中过表达所诱导的细胞生长和转移效应。这些结果共同表明，miR-210-3p 通过靶向 FGFRL1 在体外和体内调节膀胱癌的生长和转移中发挥着重要作用。

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参考信息
https://pubmed.ncbi.nlm.nih.gov/28861329/

https://pubpeer.com/publications/7C1F6E52442DD0565B0916316243DA#0

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